Tritirachium album proteinase K
proteinase K for tritirachium album
CAS: 39450-01-6;102925-54-2
Molecular Formula: C29H27N2O12P
Tritirachium album proteinase K - Names and Identifiers
Tritirachium album proteinase K - Physico-chemical Properties
| Molecular Formula | C29H27N2O12P |
| Molar Mass | 626.504641 |
| Water Solubility | Miscible with water. |
| Solubility | H2O: 10mg/mL, slightly hazy, brown-yellow |
| Appearance | powder |
| Color | white |
| PH | pH7.5～12 |
| Storage Condition | 2-8°C |
| MDL | MFCD00132129 |
| Physical and Chemical Properties | Solubility: H2O: 10 mg/mL, light hazy, brown-yellow storage conditions: 2-8℃ WGK Germany:1 |
| Use | Inactivation of nucleotidase proteins during DNA and RNA isolation. Removal of endotoxin constraints on cationic proteins, such as lysozyme and ribonuclease. Research reports show that enzymes on cell membranes used to isolate liver, yeast, and mung bean mitochondria are used for antibody labeling, and antigen binding sites are exposed in paraffin-embedded tissue sections. Proteins in Digested Brain Tissue Samples for the Study of Infectious Spongiform Encephalopathy (TSE) of Prions |
Tritirachium album proteinase K - Risk and Safety
| Hazard Symbols | Xn - Harmful |
| Risk Codes | R36/37/38 - Irritating to eyes, respiratory system and skin. R42 - May cause sensitization by inhalation |
| Safety Description | S23 - Do not breathe vapour. S24 - Avoid contact with skin. S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36/37 - Wear suitable protective clothing and gloves. S22 - Do not breathe dust. S45 - In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.) |
| WGK Germany | 1 |
| FLUKA BRAND F CODES | 10 |
| HS Code | 35079090 |
Tritirachium album proteinase K - Reference
| Reference Show more | 1. Yin Jialu, Tang Yuqian, Ren Jie, etc. Biodegradation characteristics of aflatoxin B1 by Rhodococcus lactis PD630 [J]. China food additive, 2020, 031(002):39-46. 2. Chang Zhiyuan, Xu Meiling, Wen Tingting, etc. Establishment of PCR method for detection of common foreign genes in genetically modified foods [J]. Journal of Changchun University of Science and Technology (Natural Science Edition), 2019, 042(002):137-142. 3. Gao Zhaojian, Zhang Yanqiu, Song Yulin, Zhao Yifeng. Purification and antibacterial properties of bacteriocins from Lactobacillus fermentum selected from pickled vegetables [J]. Science and Technology of food industry, 2021,42(03):201-207 283. 4. Gao Zhaojian, Wang Qiufen, Hu Xinqiang, Song Yulin, Zhao Yifeng, Chen Teng. Isolation, identification and antibacterial properties of antimicrobial lipopeptides from Bacillus coagulans XZQ-16 [J]. Science and Technology of food industry, 2021,42(03):36-42. 5. Sun Xiaohan, Sun Honghao, Chen xiucu, Lu Fujun. Screening of Lactobacillus plantarum with antibacterial activity and characteristics analysis of antibacterial substances [J]. Pig raising, 2020(06):18-20. 6. Jiang Yuhang, Li Hongwei, Yang Xiaojie, Lin lianbing, Zhang Qilin. Screening and antibacterial properties of bacteriocin-producing bacteria from the intestine of Dendrolimus punctatus [J]. Microbiology, 2021,48(01):123-134. 7. Guoqiang Cui, Xuebing Yang, Tingting Liu, Zaizhi Liu, Lei Yang,An efficient approach for the enzyme-enhanced extraction of camptothecin and 10-hydroxycamptothecin from the samara of Camptotheca acuminata using an ionic liquid solution,Separation and Puri 8. [IF=5.833] Chen Jing et al."A composite prepared from MnO2 nanosheets and a deep eutectic solvent as an oxidase mimic for the colorimetric determination of DNA."Microchim Acta. 2020 Jan;187(1):1-7 9. [IF=4.952] Li Li et al."The adhesion of the gut microbiota to insoluble dietary fiber from soy hulls promoted the proliferation of probiotics in vitro."Lwt Food Sci Technol. 2022 Jan;153:112560 10. [IF=4.098] Tao Liu et al."Rational design of a fluorescent probe for the detection of LAP and its application in drug-induced liver injury."Spectrochim Acta A. 2021 Apr;251:119362 |
Tritirachium album proteinase K - Reference Information
| introduction | protease k is a powerful proteolytic enzyme isolated from candida albicans. it has high specific activity and is a key reagent for DNA extraction. The enzyme is active in a wide pH range (4-12.5) and high temperature (50-70°C), and is used for the separation of plasmid or genomic DNA and RNA. |
| Properties | Protease K can digest natural keratin (Kerati), which is named after protease K. The main cleavage sites are carboxyl-terminal peptide bonds of aliphatic and aromatic amino acids. Due to its extensive cutting ability, it is very commonly used in laboratories. This protease belongs to the S8 family of serine protease SB superfamily (subtilisin also belongs to this superfamily). The molecular weight of protease K is 28.9 kilodaltons. |
| enzyme activity | enzyme activity of protease k,>30U/mg. At 37°C, using hemoglobin as a substrate, the amount of protease K equivalent to 1 micromole of tyrosine Folin-positive amino acid or polypeptide can be produced within 1 minute, which is defined as the activity of a unit protease K. It is effective in a wide pH range, the effective pH range is pH 4.0-12.5, and the optimal pH range is pH 7.5-8.0. The optimal reaction temperature of protease K is 65 ℃, but protease K itself degrades very quickly at 65 ℃ or higher. In many cases, the reaction temperature is 50 -55 ℃. |
| Application | Protease K is often used in the field of molecular biology. In the preparation of nucleic acid, protease K can remove the heteroprotein and quickly inactivate the nucleic acid enzyme to avoid its degradation of the target product DNA and RNA. In addition, protease K has other advantages. It can still retain activity in the presence of a variable agent, such as SDS, urea, EDTA, etc., which is quite different from other proteases (such as trypsin, chymotrypsin). |
| Use | Protease K is named protease K because it can digest keratin. Protease K family contains various intracellular peptidases secreted by fungi, yeast and gram-negative bacteria, among which the enzymes secreted by bacteria have a high degree of sequence similarity (>55%). The protease K secreted by Tritirachiumalbum Limber is the model protein of this family. Protease K has cleavage activity on most substrates, but it prefers peptide chains with aliphatic and aromatic hydrocarbons. It belongs to serine protease and has a typical serine active site: Asp39-His69-Ser224. It contains a single peptide chain containing 277 amino acid sites, and has 2 disulfide bonds, a vacant cysteine site and two calcium ion binding sites. The application of protease K is mainly to remove DNA enzyme and RNA enzyme during nucleic acid extraction, and can remove histones bound to the genome, and can also be used for inactivation of alkaline phosphatase. At present, molecular biology technology has been applied to many detection standards, such as transgenic detection, animal attribute detection, pathogenic microorganism detection, etc. The extraction quality of nucleic acid in molecular biological detection directly affects the amplification efficiency of subsequent PCR reactions. Many standards set up a step of adding protease K to digest in the nucleic acid extraction step in order to extract purer nucleic acids. In order to protect nucleic acid, molecular biology nucleic acid extraction experiments usually contain EDTA and other reagents. Because EDTA can be complexed with metal ions, it may affect the enzyme activity; and it is usually digested at pH 8.0 and 55 ℃. Different pH and temperature conditions will affect the enzyme activity. Inactivation of nucleotidase proteins during DNA and RNA isolation. Removal of endotoxin constraints on cationic proteins, such as lysozyme and ribonuclease. Research reports show that enzymes on cell membranes used to isolate liver, yeast, and mung bean mitochondria are used for antibody labeling, and antigen binding sites are exposed in paraffin-embedded tissue sections. Digestion of proteins in brain tissue samples for the study of prion infectious spongiform encephalopathy (TSE) |
Last Update：2024-04-09 20:52:54
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